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OxyPet Case Study 4 Blog: Nano-Bubble Oxygen Water Stability Over Time and Temperature

Updated: 7 days ago

By Lindsey John Taylor, Co-Founder of OxyPet


Why Stability Matters — and What This Case Study Could Mean for Dogs & Cats

When you’re working with something as powerful (and tiny) as nano-bubble oxygen, one of the biggest questions is: Does it last? Can it handle heat? Will it still work months — or even years — later?

So it was tested. And the results were clear.

This case study confirms that OxyPet’s nano-bubble oxygen particles remain stable, bioavailable, and effective, even after exposure to boiling temperatures and long-term storage.

That means OxyPet doesn’t just work — it could continue to support hydration and cellular oxygen delivery regardless of how or when you use it.


 

🧪 Case Study Overview

Conducted by: Caroline Mansfield (Dip. Nat) Method: Live blood microscopy Focus: Measuring changes in blood quality and flow after consuming OxyPet from bottles subjected to shelf-life and heat testing.

🧬 Importantly, this test was carried out on human blood—not animals—as part of our commitment to protecting animal welfare, so there is no invasive testing. 

 

🧊 Stability Over Time We tested a sealed bottle of OxyPet that had been manufactured and stored for 18 months.

After consumption, the blood still showed the same improvements seen in all other human case studies:

  • Free-flowing red blood cells

  • No rouleaux (clumping)

  • No fibrinogen (inflammation marker)

  • Clear, healthy membrane definition

✅ Result: The nano-oxygen remained intact, suspended, and functional, showing no degradation with time.

 

♨️ Stability Under Heat

We also tested whether OxyPet’s nano-oxygen particles could survive extreme heat — something most oxygen waters can’t do.

OxyPet was heated to 100°C (boiling point) for:

  • 1 minute

  • 2 minutes

  • 5 minutes

After cooling, the water was consumed, and blood was re-analysed using live microscopy.

✅ Result: No loss of benefit. Blood flow, structure, and quality remained consistent. The oxygen nanoparticles maintained their shape and potential.

 

Oxygen Water for Dogs, Practical Use: Heat, Storage, and Homemade Additions

Whether you’re:

  • Storing OxyPet in the cupboard long-term

  • Using it while travelling or in hot conditions

  • Warming it gently before feeding

  • Or incorporating it into daily feeding routines…

You can trust that the oxygen remains stable and effective.

OxyPet can work beautifully for all feeding styles — whether you:

  • Prepare home-cooked or organic meals

  • Feed raw, lightly cooked, or dry food

  • Use broths, toppers, or therapeutic blends and teas

You can also safely use it in:

  • Organic bone broths

  • Frozen treats or cubes

  • Nutritional teas, gravies, or infused recipes

  • Hydration mixes for senior or sensitive dogs

 

Could This Help Dogs Absorb Nutrients Better?

Because the nano-bubble oxygen in OxyPet is absorbed so rapidly, it may supercharge the way nutrients are transported in the body. This could:

  • Support digestion and nutrient absorption

  • Help cells get what they need faster

  • Reduce stress on the gut

  • Enhance recovery, detox, and energy

More than just hydration — it could help the whole body perform more efficiently, especially when combined with nutritious meals.

 

🧠 Final Thought

This case study adds another layer of confidence in OxyPet—not just for its function but also for its stability as an oxygen water for dogs

The nano-bubble oxygen remained effective after boiling temperatures and after sitting unopened for over a year. That’s rare. Most oxygenated waters simply can’t do that, but then they don't have their oxygen as nano-bubbles.

(Independent human blood microscopy study on OxyPet stability in time and heat)

 

Disclaimer:

This article is for informational and educational purposes only. It does not constitute medical advice. Everything shared here is purely educational, based on independent findings and lived experience. The potential benefits described are based on preliminary research findings. As always, check in with your integrated veterinary professional if your dog or cat has a health condition. References:

  1. Vatnehol SAS, Hol PK, Bjørnerud A, Amiry-Moghaddam M, Haglerød C, Storås TH. Effect of Drinking Oxygenated Water Assessed by in vivo MRI Relaxometry. J Magn Reson Imaging. 2020;52(3):720–8. 🔗 https://pubmed.ncbi.nlm.nih.gov/32100358/

  2. Forth W, Adam O. Uptake of oxygen from the intestine—experiments with rabbits. Eur J Med Res. 2001 Nov 20;6(11):488–92. 🔗 https://pubmed.ncbi.nlm.nih.gov/32100358/

  3. Fang CH, Tsai CC, Shyong YJ, Yang CT, Li KY, Lin YW, et al. Effects of Highly Oxygenated Water in a Hyperuricemia Rat Model. J Healthc Eng. 2020 Jan 30;2020:1323270. 🔗 https://pubmed.ncbi.nlm.nih.gov/32076494/

  4. Pittman RN. Oxygen Transport. Regulation of Tissue Oxygenation. Morgan & Claypool Life Sciences; 2011 [cited 2022 Nov 26]. 🔗 https://www.ncbi.nlm.nih.gov/books/NBK54103/

  5. Hypoxia - StatPearls - NCBI Bookshelf [Internet]. [cited 2022 Dec 15]. 🔗 https://www.ncbi.nlm.nih.gov/books/NBK482316/

  6. Khoo J, Hagemeyer CE, Henstridge DC, Kumble S, Wang TY, Xu R, et al. Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomised placebo-controlled trial. PLoS ONE. 2021 Jul 14;16(7):e0254619. 🔗 https://pubmed.ncbi.nlm.nih.gov/34260650/

  7. Moen I, Ugland H, Strömberg N, Sjöström E, Karlson A, Ringstad L, et al. Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5. Health Sci Rep. 2018 Jun 13;1(7):e57. 🔗 https://pubmed.ncbi.nlm.nih.gov/30623087/

  8. Honary S, Zahir F. Effect of Zeta Potential on the Properties of Nano-Drug Delivery Systems - A Review (Part 1). Trop J Pharm Res. 2013 May 9;12(2):255–64. 🔗 https://pubmed.ncbi.nlm.nih.gov/39369767/

  9. Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres M del P, Acosta-Torres LS, et al. Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnology. 2018 Sep 19;16(1):71. 🔗 https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-018-0392-8

  10. Handajani YS, Tenggara R, Suyatna FD, Surjadi C, Widjaja NT. The effect of oxygenated water in Diabetes Mellitus. Med J Indones. 2009 May 1;18(2):102–7. 🔗 https://mji.ui.ac.id/journal/index.php/mji/article/view/351

  11. Aoki K, Ida Y, Fukushima N, Matsumura H. Topical application of oxygen nano-bubble water enhances the healing process of ischaemic skin wound healing in an animal model. Int Wound J. 2022 Apr 10;19(7):1843–52. 🔗 https://pubmed.ncbi.nlm.nih.gov/35403362/

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