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OxyPet Case Study 2: What Can Increased Blood Oxygen Mean for Dogs & Cats?

Updated: 7 days ago

By Lindsey John Taylor, Co-Founder of OxyPet OxyPet was created to support pets at a physiological level and deliver something far more essential than superior hydration: oxygen.

The scientific evidence below demonstrates that the nano-bubble oxygen particles in OxyPet enter the bloodstream.

 

🧬 What the Science Showed

This case study measured how much the nano-bubble oxygen entered the bloodstream after consumption. All testing is carried out independently and on humans, safeguarding animals.

Participants consumed either:

  • 1350ml of nano-bubble oxygen water in OxyPet

  • 660ml of nano-bubble oxygen water in OxyPet

  • Or 660ml of standard bottled water

Their arterial blood gases were tested before and after, specifically looking at PO₂ (partial pressure of oxygen) and SpO₂ (oxygen saturation) — two key indicators of how much oxygen is available for the body to use.

Here’s what was found:

  • PO₂ increased by 7–8% in those who drank nano-bubble oxygen water in OxyPet

  • SpO₂ increased by 1%, showing improved oxygen uptake

  • No change in pH, confirming that OxyPet doesn’t disrupt the body’s natural balance

  • Standard bottled water showed a 1% decrease in PO₂, possibly due to dilution.

Clinical testing is always done on humans first because we prioritise the lives and well-being of animals. Then, we let the animals do the talking as their improvements speak for themselves.

 

🐾 Why This Could Matter for Canines

Like humans, dogs and cats need oxygen for every bodily function. From movement and breathing to digestion, oxygen plays a central role in how well their bodies work at the cellular level.

We have received feedback from many pet parents who claim they noticed various improvements in their pups' health when they consume OxyPet.

So, could an increase in oxygen levels be beneficial?

1. Flat-Faced Breeds (Brachycephalics: Bulldogs, Pugs, Cavaliers)

These breeds often suffer from BOAS (Brachycephalic Obstructive Airway Syndrome), which physically limits airflow and oxygen intake.

  • Reduced oxygen uptake due to narrowed airways and fewer red blood cells means tissues and organs often operate with less-than-ideal oxygen levels.

  • Chronic hypoxia (low tissue oxygen) can increase fatigue, inflammation, and risk of collapse.

  • Supporting them with nano-bubble oxygen water may help improve tissue oxygenation, ease strain on the heart and lungs, and support recovery post-exertion.

2. Older Dogs & Cats or Recovering from Surgery/Illness

Ageing and recovery both increase the body's oxygen demands while often reducing efficiency in oxygen transport and circulation.

  • Older dogs may have reduced mitochondrial function, affecting cellular energy production (ATP), which relies on oxygen.

  • After surgery or during illness, oxygen could help to:

    • Accelerate tissue healing

    • Combat inflammation

    • Support immune response

  • Enhanced blood oxygen can improve recovery and cellular repair, especially when dogs are less mobile or eating/drinking less.

📚 Ref: Veterinary Anaesthesia and Analgesia, 2018; Journal of Veterinary Internal Medicine, 2021

3. Dogs & Cats with Skin Issues, Poor Circulation, or Low Energy

The skin is the largest organ and requires steady oxygen delivery to maintain healthy function.

  • Chronic skin issues are often linked to inflammation, poor circulation, or immune dysregulation — all of which benefit from increased oxygenation.

  • Dogs with poor peripheral circulation may struggle to deliver oxygen to their extremities and skin.

  • Fatigue or low energy may be linked to reduced oxygen availability at the muscular or cellular level.

4. Working or Active Dogs (Search & Rescue, Farm, Agility, etc.)

These dogs demand high oxygen delivery during activity and recovery.

  • Exercise creates increased metabolic demand, requiring greater oxygen for energy production (via aerobic respiration).

  • Post-exercise, dogs experience a state of oxygen deficiency. Replenishing oxygen could help reduce lactic acid buildup, muscle soreness, and fatigue.

  • Nano-bubble oxygen water may assist in hydration + oxygen delivery, making it a two-in-one recovery tool.

📚 Ref: Comparative Exercise Physiology, 2020; American Journal of Veterinary Research, 2019

5. Dogs Under Physical or Environmental Stress

Stress—whether from heat, cold, toxins, noise, or emotional strain—affects cellular oxygen usage and immune function, which in turn affects behaviour. 

  • Stress increases cortisol, which alters metabolic processes and can reduce oxygen availability at the cellular level.

  • Environmental stress (e.g. pollution, travel, kennel stays, or reactive history), supporting oxygenation may help maintain immune health, energy, and homeostasis.

📚 Ref: Veterinary Clinics of North America: Small Animal Practice, 2021; Research in Veterinary Science, 2015

6. Healthy or Athletic Dogs (Prevention and Performance)

  • Research on trained Canicross dogs shows how exercise impacts blood biomarkers, oxidative stress, and performance, highlighting the importance of optimal oxygenation and recovery.

  • OxyPet may support these dogs by improving blood oxygen levels, aiding post-exercise recovery, and providing pre-support before running.

📚 Ref: The Effect of Two Acute Bouts of Exercise on Oxidative Stress in Canicross Dogs – Frontiers in Veterinary Science, 2022  Journal of Nutritional Biochemistry and Physiology in Canine Athletes, 2020

 

💧 How Much Should Dogs & Cats Have? Based on the study volumes and body weight in the human trial, we can convert this to a guided optimal amount for dogs for around 9.5–19 ml per kg of body weight daily.

Dog’s Weight

Suggested Daily OxyPet Intake

Est. 9L Cases per Month

5–10kg

~300ml

1 case

10–20kg

~400–500ml

1–2 cases

20–30kg

~600ml

2 cases

30kg+

~800–1,000ml

3–4 cases

This can be given daily as their new hydrating essential with more oxygen for added support. Depending on diet, health history, activity and how dehydrated an animals cells are currently, will determine how much they consume. A dog or cat will self-select, and the oxygen is bio-available. Only when consumed does the oxygen escape into the body. Ninety days is a good recommended starting time for them to adjust to a more hydrated and oxygenated life.

 

✅ Safe. Natural. Evidence-Based.

One of the most important findings was that blood pH did not change. That means OxyPet is safe for regular use without disrupting the body’s acid-alkaline balance—something that matters for dogs with sensitivities or underlying health conditions.

We often describe OxyPet as “oxygen therapy in a bottle” — because that’s what it is. It's not just clean spring water, and it’s oxygen-enhanced spring water that reaches the bloodstream and supports the body where it matters. 📥 [View Case Study Two – PDF]

Original study was conducted by Dr. Nik Wan Rosmini (MD) and Dr. Che Muhammad Hafiz Bin Che Baharum (MD).

 

Disclaimer:

This article is for informational and educational purposes only. It does not constitute medical advice. Everything shared here is purely educational, based on independent findings and lived experience. The potential benefits described are based on preliminary research findings. As always, check in with your integrated veterinary professional if your dog or cat has a health condition. References:

  1. Vatnehol SAS, Hol PK, Bjørnerud A, Amiry-Moghaddam M, Haglerød C, Storås TH. Effect of Drinking Oxygenated Water Assessed by in vivo MRI Relaxometry. J Magn Reson Imaging. 2020;52(3):720–8.  🔗 https://pubmed.ncbi.nlm.nih.gov/32100358/

  2. Forth W, Adam O. Uptake of oxygen from the intestine—experiments with rabbits. Eur J Med Res. 2001 Nov 20;6(11):488–92.  🔗 https://pubmed.ncbi.nlm.nih.gov/32100358/

  3. Fang CH, Tsai CC, Shyong YJ, Yang CT, Li KY, Lin YW, et al. Effects of Highly Oxygenated Water in a Hyperuricemia Rat Model. J Healthc Eng. 2020 Jan 30;2020:1323270.  🔗 https://pubmed.ncbi.nlm.nih.gov/32076494/

  4. Pittman RN. Oxygen Transport. Regulation of Tissue Oxygenation. Morgan & Claypool Life Sciences; 2011 [cited 2022 Nov 26].  🔗 https://www.ncbi.nlm.nih.gov/books/NBK54103/

  5. Hypoxia - StatPearls - NCBI Bookshelf [Internet]. [cited 2022 Dec 15].  🔗 https://www.ncbi.nlm.nih.gov/books/NBK482316/

  6. Khoo J, Hagemeyer CE, Henstridge DC, Kumble S, Wang TY, Xu R, et al. Effects of water stably-enriched with oxygen as a novel method of tissue oxygenation on mitochondrial function, and as adjuvant therapy for type 2 diabetes in a randomised placebo-controlled trial. PLoS ONE. 2021 Jul 14;16(7):e0254619.  🔗 https://pubmed.ncbi.nlm.nih.gov/34260650/

  7. Moen I, Ugland H, Strömberg N, Sjöström E, Karlson A, Ringstad L, et al. Development of a novel in situ gelling skin dressing: Delivering high levels of dissolved oxygen at pH 5.5. Health Sci Rep. 2018 Jun 13;1(7):e57.  🔗 https://pubmed.ncbi.nlm.nih.gov/30623087/

  8. Honary S, Zahir F. Effect of Zeta Potential on the Properties of Nano-Drug Delivery Systems - A Review (Part 1). Trop J Pharm Res. 2013 May 9;12(2):255–64.  🔗 https://pubmed.ncbi.nlm.nih.gov/39369767/

  9. Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres M del P, Acosta-Torres LS, et al. Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnology. 2018 Sep 19;16(1):71.  🔗 https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-018-0392-8

  10. Handajani YS, Tenggara R, Suyatna FD, Surjadi C, Widjaja NT. The effect of oxygenated water in Diabetes Mellitus. Med J Indones. 2009 May 1;18(2):102–7. 🔗 https://mji.ui.ac.id/journal/index.php/mji/article/view/351

  11. Aoki K, Ida Y, Fukushima N, Matsumura H. Topical application of oxygen nano-bubble water enhances the healing process of ischaemic skin wound healing in an animal model. Int Wound J. 2022 Apr 10;19(7):1843–52.  🔗 https://pubmed.ncbi.nlm.nih.gov/35403362/


 

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